Pengaruh Penggunaan Metode Lactic Acid-Sucrose dengan Microwave Assisted Extraction (MAE) terhadap Polifenol Total dari Herba Suruhan (Peperomia pellucida (L.) Kunth)

  • Kharisma Ayu Tandi Datu
  • Nurul Fitriani
  • Islamudin Ahmad

Abstract

This study aim to determine the effect of use lactic acid and sucrose with assistance of Microwave Assisted Extraction (MAE) on Total Polyphenols from Herbs Suruhan (Peperomia pellucida (L.) Kunth). The dried sample of Herbs Suruhan was extracted using (green solvent) lactic acid-sucrose with a ratio of 1:1 g/g, 2:1 g/g and 3:1 g/g and the extraction conditions microwave strength was 50% for 10 minutes at a ratio sample and solvent 1:10 g/ml. Then the extract solution obtained to determine the total polyphenol level using the Folin Ciocalteu reagent and it absorbance was measured using a UV-VIS spectrophotometer at a wavelength of 791 nm. Based on the result of the study, obtained levels of polyphenols from each solvent condition used on the sample were 573,443 mg GAE/g (2:1), 472,479 mg GAE/g (1:1) and 420,14 mg GAE/g (3:1). It can be concluded that the lactic acid-sucrose method with microwave assisted extraction can be used to extract polyphenol compounds from Herbs Suruhan.

References

[1] Raghavendra H. L. and P. K. T. R. (2018) ‘Ethnobotanical Uses, Phytochemistry and Pharmacological Activities of Peperomia Pellucida (L.) Kunth (Piperaceae)-a Review’, International Journal of Pharmacy and Pharmaceutical Sciences, 10(2), p. 1.
[2] Majumder, P. (2011) ‘Phytochemical, Pharmacognostical and Physicochemical Standardization of Peperomia pellucida (l.) HBK. Stem’, Pharmacie Globale, 8 (06).
[3] Oloyede, G. K., Onocha, P. A. and Olaniran, B. B. (2011) ‘Phytochemical, toxicity, antimicrobial and antioxidant screening of leaf extracts of Peperomia pellucida from Nigeria’, Advances in Environmental Biology, 5(12), pp. 3700–3709.
[4] Bialangi, N., Mustapa, M. A., Salimi,Y. K., Widiantoro, A. and Situmeang, B. (2016) ‘Antimalarial activity and phitochemical analysis from Suruhan (Peperomia pellucida) extract’, Jurnal Pendidikan Kimia, 8(3), pp. 183–187.
[5] Hemalatha, S., Mandal, V. and Mohan, Y. (2007) ‘Microwave assisted extraction - An innovative and promising extraction tool for medicinal plant research’, Pharmacognosy Reviews, 1(1), pp. 7–18.
[6] Handayani, D. (2014) ‘Optimasi Ekstraksi Ampas Teh Hijau (Camellia sinensis) Menggunakan Metode Microwave Assisted Extraction Untuk Menghasilkan Ekstrak Teh Hijau’, Traditional Medicine Journal, 19(January), pp. 29–35.
[7] Fernández, M., Espino, M., Gomez, F., & Silva, M. (2017). Novel approaches mediated by tailor-made green solvents for the extraction of phenolic compounds from agro-food industrial by-products. Food Chemistry, 239, 671-678.
[8] Dai, Y., van Spronsen, J., Witkamp, G., Verpoorte, R. and Choi,Y. H. (2013) ‘Natural deep eutectic solvents as new potential media for green technology’, Analytica Chimica Acta, 766, pp. 61–68.
[9] Wei, Z., Qi, X., Li, T., Luo, M., Wang, W., Zu, Y. and Fu, Y. (2015). Application of natural deep eutectic solvents for extraction and determination of phenolics in Cajanus cajan leaves by ultra performance liquid chromatography. Separation and Purification Technology, 149, 237-244.
Published
2019-10-31
How to Cite
Datu, K. A. T., Fitriani, N., & Ahmad, I. (2019). Pengaruh Penggunaan Metode Lactic Acid-Sucrose dengan Microwave Assisted Extraction (MAE) terhadap Polifenol Total dari Herba Suruhan (Peperomia pellucida (L.) Kunth). Proceeding of Mulawarman Pharmaceuticals Conferences, 10(1), 114-117. https://doi.org/10.25026/mpc.v10i1.373

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