Ekstraksi Polifenol Total dari Umbi Bawang Dayak (Eleutherine bulbosa [Mill.] Urb.) menggunakan Metode Lactic Acid-Sucrose based Microwave Assisted Extraction

  • Kurnya Kurnya
  • Hajrah Hajrah
  • Islamudin Ahmad


Dayak Onion (Eleutherine bulbosa [Mill.] Urb.) It is a plant that grows in Indonesia precisely in the Kalimantan area and has traditionally efficacious in curing various diseases such as diabetes mellitus, hypertension, breast cancer, cholesterol-lowering, and stroke. This study aims to determine the effect of using the lactic acid-sucrose based microwave-assisted extraction (MAE) method to extract total polyphenols from Dayak onion tubers (Eleutherine bulbosa [Mill.] Urb.). Dry samples were extracted using a lactic-sucrose acid (green solvent) solvent in the ratio of 1: 1, 2: 1, and 3: 1  g/g with 50% microwave strength for 10 minutes with a ratio of samples and solvents of 1:10 g /mL. Extracts that have been obtained then determined the total polyphenol levels using a UV-VIS spectrophotometer at a wavelength of 791 nm. Based on the results of the study, it was obtained the levels of polyphenols from each condition of the solvent used in the sample that is 28.73 mg GAE/g sample ( 1: 1 g/g solvent); 20.2 mg GAE/g sample ( 2:1 g/g solvent); 36.35 mg GAE/g sample ( 3:1 g/g solvent). From this study, it can be concluded that the MAE method can be used to extract polyphenol compounds from Dayak bulbs.


[1] Syamsul, E.S., dan Supomo, 2013. “Pengembangan Kearifan Lokal Ekstrak Umbi Bawang Tiwai (Eleutherine palmifolia (L.) Merr) Dalam Bentuk Sediaan Granule Effervescent Sebagai Food Supplement”. Laporan Hasil Penelitian Terapan. Kalimantan Timur. U.P.B. Science Bulletin.
[2] Kuntorini, E.M. and Nugroho, L.H. 2010. Structural development and bioactive content of red bulb plant (Eleutherine americana: a traditional medicines for local Kalimantan people).
[3] Arung, E.T., Kusuma, I.W., Christy, E.O., Shimizu, K. and Kondo, R. 2009. Evaluation of medicinal plants from Central Kalimantan for antimelanogenesis. Journal of Natural Medicines.
[4] Noorcahyati. 2012. Tumbuhan Berkhasiat Obat Etnis Asli Kalimantan. Balikpapan: Badan Penelitian Teknologi Konservasi Sumber Daya Alam. Polyphenols, Intracellular Signaling and Inflammation. Ann Ist Super Sanita.
[5] Mierza, Vriezka, Suryanto, Dwi, dan Nasution, M. Pandapotan. 2011. Skrining Fitokimia dan Uji Efek Antibakteri Ekstrak Bawang Sabrang (Eleutherine palmifolia Merr.), Prosiding Seminar Nasional Biologi. Medan: USU Press.
[6] Nascimento, M.S., Vieira, J.M.S., Malheiros, L. C. S., Junior, J.O.C. Silva, Rodrigues, L.C.S., and Barbosa, W.L.R. 2012. Characterisation of Isoeleutherin in Aqueous Extract of Eleutherine plicata Herb, Iridaceae, Active against Entamoeba hystolica/Entamoeba dispar In Vitro. International Journal of Pharmaceutical Sciences adn Research.
[7] Saleh, Chairul. 2010. Uji Hipoglikemik Ekstrak Etanol Umbi Eleutherine americana Merr. Mulawarman Scientific.
[8] Pratiwi, Dina, Wahdaningsih, Sri, and Isnindar. 2013. The Test of Antioxidant Activity from Bawang Mekah Leaves (Eleutherine americana Merr.) Using DPPH (2,2-Diphenyl-1-picrylhydrazyl) Method. Traditional Medicine Journal.
[9] Santangelo, Carmela, Vari, Rosaria, Scazzocchio, Beatrice, Benedetto, Roberta Di, Filesi, Carmela, and Masella, Roberta. 2007. Polyphenols, Intracellular Signaling and Inflammation. Ann Ist Super Sanita.
[10] Biswas, A.; Shogren, R. L.; Stevenson, D. G.; Willett, J. L.; Bhowmik, P. K. 2006. Ionic liquids as solvents for biopolymers: Acylation of starch and zein protein. Carbohydr. Polym.
[11] Heleno, S. A., M. A. Prieto, L. Barros, A. Rodrigues, M. F. Barreiro, dan I. C. F. R. Ferreira. 2016. “Optimization of MicrowaveAssisted Extraction of Ergosterol from Agaricus Bisporus L. by-Products Using Response Surface Methodology”. Food and Bioproducts Processing 100. Institution of Chemical Engineers.
[12] Chemat, F., & Cravotto, G. 2013. Microwave-assisted Extraction for Bioactive Compounds: theory and Practice. Springer. New York.
[13] Santos-Buelga, C., Gonzalez-Manzano, S., Duenas, M., and Gonzalez-Paramas, A. M. 2012. Extraction and Isolation of Phenolic Compounds. Satyajit D. Sarker and Lutfun Nahar (eds), Natural Products Isolation, Methods in Molecular Biology, Springer Science. New York.
[14] Alara, O.R., Abdurahman, N.H., Olalere, O.A., 2017. Ethanolic extraction of flavonoids, phenolics and antioxidants from Vernonia amygdalina leaf using two-level factorial design. J. King Saud Univ.
[15] Alara, O.R., Abdul Mudalip, S.K., Olalere, O.A., 2017. Optimization of mangiferin extracted from Phaleria macrocarpa fruits using response surface methodology. J. Appl. Res. Med. Aromat. Plants.
How to Cite
Kurnya, K., Hajrah, H., & Ahmad, I. (2019). Ekstraksi Polifenol Total dari Umbi Bawang Dayak (Eleutherine bulbosa [Mill.] Urb.) menggunakan Metode Lactic Acid-Sucrose based Microwave Assisted Extraction . Proceeding of Mulawarman Pharmaceuticals Conferences, 10(1), 118-121. https://doi.org/10.25026/mpc.v10i1.374

Most read articles by the same author(s)

1 2 > >>