Ekstraksi Polifenol Total dari Umbi Bawang Dayak (Eleutherine bulbosa [Mill.] Urb.) Menggunakan Metode Citric Acid-Glucose based Microwave Assisted Extraction

  • Selvy Jumiatul Astati
  • Mirhansyah Ardana
  • Islamudin Ahmad

Abstract

The use of environmentally friendly solvent-based expulsion methods today began to be widely used, one of which is a microwave-assisted extraction (MAE) using a citric acid-glucose acid solvent. The study aims to determine the influence of the use of citric acid-glucose based microwave-assisted extraction against the total extraction of polyphenols from Dayak onion bulbs (Eleutherine bulbosa [Mill.] Urb.).The dried samples were extracted using a solvent (green solvent) citric acid – glucose with a ratio of 4:1, 5:1 and 6:1 g/g with the extraction conditions in the form of microwave strength of 50% for 10 minutes with a sample and solvent ratio of 1:10 g/mL, then Extract solution obtained further determination of the total levels of polyphenols using Folin ciocalteu reagents, and its absorption was measured using a UV-vis spectrophotometer at a wavelength of 791 nm. Based on the results of the study, the total amount of polyphenols obtained from each of the solvent conditions used against the samples was 21.92 mgGAE/g samples, 13.86 mgGAE/g samples, and 14.38 mgGAE/g samples. From this study, it can be concluded that the concentration of citric acid in the microwave-based citric acid-glucose method is assisted by extraction according to the ability of the solvent in the competency of polyphenols from Dayak onion bulbs.

References

[1]
[2] Naspiah, N., Iskandar, Y., dan Moelyono, M . 2014. Review Article: Dayak Onion (Eleutherine americana Merr.), Multifunction Plant. Institut Teknologi Bandung, Universitas Padjadjaran. IJAS Vol.4 No.2.
[3] Fernández, M., Espino, M., Gomez, F., & Silva, M. 2017. Novel approaches mediated by tailor-made green solvents for the extraction of phenolic compounds from agro-food industrial by-products. Food Chemistry, 239, 671-678.
[4] Hao, J. Y., Han, W., Huang, S. D., Xue, B. Y., & Deng, X. 2002. Microwave-assisted Extraction of Artemisinin from Artemisia annua L. Separation and Purification Technology. 28.
[5] Bugallo, R.A., Segade, S.R., Gomez, E.F. 2007. Comparison of Slurry Sampling and Microwave Assisted Digestion for Calcium, Magnesium, Iron, Copper and Zinc Determination in Fish Tissue Samples by Flame Atomic Absorption Spectrometry. Talanta 72.
[6] Soylak, M., Tuzen, M., Souza, A.S., Korn, M.G.A., Ferreira, S.L.C. 2007. Optimization Of Microwave Assisted Digestion Procedure For The Determination Of Zinc, Copper And Nickel In Tea Samples Employing Flame Atomic Absorption Spectrometry. Journal of Hazardous Materials.
[7] Ahmad, I., Surya Pertiwi, A., Heryani Kembaren, Y., Rahman, A. and Mun’im, A. 2018. Application of Natural Deep Eutectic Solvent-Based Ultrasonic Assisted Extraction of Total Polyphenolic and Caffeine Content from Coffe Beans (Coffea Beans L.) For Instant Food Products. Journal of Applied Pharmaceutical Science Vol. 8(08), pp. 138-143.
[8] Wei, Z., Qi, X., Li, T., Luo, M., Wang, W., Zu, Y. and Fu, Y. 2015. Application of natural deep eutectic solvents for extraction and determination of phenolics in Cajanus cajan leaves by ultra performance liquid chromatography. Separation and Purification Technology, 149, 237-244.
Published
2019-10-31
How to Cite
Astati, S. J., Ardana, M., & Ahmad, I. (2019). Ekstraksi Polifenol Total dari Umbi Bawang Dayak (Eleutherine bulbosa [Mill.] Urb.) Menggunakan Metode Citric Acid-Glucose based Microwave Assisted Extraction. Proceeding of Mulawarman Pharmaceuticals Conferences, 10(1), 139-142. https://doi.org/10.25026/mpc.v10i1.379

Most read articles by the same author(s)

<< < 1 2 3 4