Uji Aktivitas Antibakteri Ekstrak Kombinasi Umbi Bawang Tiwai dan Kulit Buah Lemon Terhadap Pertumbuhan Staphylococcus aureus dan Escherichia coli

Antibacterial Activity Test of Combination of Tiwai Onion Bulbs and Lemon Peel on the Growth of Staphylococcus aureus and Escherichia coli

Authors

  • Adinda Niti Meilaningrum Laboratorium Penelitian dan Pengembangan Kefarmasian “Farmaka Tropis”, Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia
  • Novita Eka Kartab Putri Laboratorium Penelitian dan Pengembangan Kefarmasian “Farmaka Tropis”, Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia
  • Yurika Sastyarina Laboratorium Penelitian dan Pengembangan Kefarmasian “Farmaka Tropis”, Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

DOI:

https://doi.org/10.25026/mpc.v13i1.436

Keywords:

Tiwai onion, lemon rind, antibacterial, well diffusion, E. coli, S. aureus

Abstract

Tiwai onion (Eleutherine americana (Aubl.) Merr) is a plant that contains several groups of secondary metabolites, one of which is the alkaloid group. Lemon rind (Citrus limon) is a plant that contains more than 2.5% essential oil and hesperidin. Several classes of secondary metabolite compounds from these two plants have antibacterial activity. This study aims to determine the characteristics of the extract and the antibacterial activity of the extract combination. The extract characteristics were tested in the form of organoleptic, pH and solubility. Meanwhile, the antibacterial activity test was carried out by using the well diffusion method. The results obtained were the characteristics of the tiwai bulb extract and lemon peel in the form of organoleptic (color: blackish red and yellow, thick texture, distinctive aroma of onion bulbs and lemon peel); pH (6.4 and 4.8); solubility (soluble in ethanol, methanol and aquades) and antibacterial activity at a concentration of 12: 3 with an average of 8.75mm (S. aureus) and 7mm (E.coli); 12: 6 with a mean of 11mm (S.aureus) and 7.25mm (E.coli); 12: 8 with an average of 12.25mm (S.aureus) and 13.75mm (E.coli). It can be concluded that at a concentration of 12: 8 the combination of these extracts had the highest average inhibition zone in inhibiting the two bacteria.

References

[1] Pratiwi. 2008. Mikrobiologi Farmasi. Jakarta: Erlangga.

[2] Jawetz et al. 2001. Mikrobiologi Kedokteran. Penerbit BK. Kedokteran. Jakarta: EGC. Hal: 609-610, 627-629, 638-639.

[3] Igoli, J. O et al. 2000. Folk Medicines of The Lower Benue Valley of Nigeria In : Recent progression in Medicinal plants, Ethnomedicine and Pharmacognosypart II, 1, 327-338.

[4] Widayat dkk. 2016. Aktivitas Ekstrak Bawang Tiwai (Eleutherine americana (Aubl.) Merr.) Terhadap Bakteri Salmonella thyposa. Prosiding Seminar Nasional Tumbuhan Obat Indonesia Ke-50.

[5] Tanjung K dkk. 2008. Efektivitas Ekstrak Kulit Jeruk Lemon (Citrus limonum) Terhadap Daya Hambat Pertumbuhan Aeromonas hydrophila Secara In Vitro. Jurnal Berkala Ilmiah Perikanan Vol. 3, No.1.

[6] Dewatisari, W. F. 2020. Perbandingan Pelarut Kloroform Dan Etanol Terhadap Rendemen Ekstrak Daun Lidah Mertua (Sansevieria trifasciata. Prain) Menggunakan Metode Maserasi. In Prosiding Seminar Nasional Biologi (Vol. 6, No. 1, pp. 127-132).

[7] Nurhayati, L. S., Yahdiyani, N., & Hidayatulloh, A. 2020. Perbandingan Pengujian Aktivitas Antibakteri Starter Yogurt Dengan Metode Difusi Sumuran Dan Metode Difusi Cakram. Jurnal Teknologi Hasil Peternakan, 1(2), 41-46.

[8] Zeniusa, P., Ramadhian, M. R., Nasution, S. H., & Karima, N. 2019. Uji Daya Hambat Ekstrak Etanol Teh Hijau Terhadap Escherichia coli Secara In Vitro. Jurnal Majority, 8(2), 136-143.

[9] Azizah, M., & Ekawati, S. 2019. Profil Kromatogram dan Uji Aktivitas Antibakteri Beberapa Fraksi Ekstrak Daun Kemuning (Murraya paniculata (L.) Jack) terhadap Bakteri Penyebab Disentri dengan Metode Difusi Agar. Jurnal Penelitian Sains, 19(2), 86-9.

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Published

2021-04-10

How to Cite

Meilaningrum, A. N., Putri, N. E. K., & Sastyarina, Y. (2021). Uji Aktivitas Antibakteri Ekstrak Kombinasi Umbi Bawang Tiwai dan Kulit Buah Lemon Terhadap Pertumbuhan Staphylococcus aureus dan Escherichia coli: Antibacterial Activity Test of Combination of Tiwai Onion Bulbs and Lemon Peel on the Growth of Staphylococcus aureus and Escherichia coli. Proceeding of Mulawarman Pharmaceuticals Conferences, 13(1), 8–13. https://doi.org/10.25026/mpc.v13i1.436