Uji Kualitatif Aktivitas Radikal Bebas DPPH dari Daun Penggugut (Knema pallens W.J.deWilde)
Qualitative Test of DPPH Radical Scavenging Activity from Penggugut Leaves (Knema pallens W.J.deWilde)
DOI:
https://doi.org/10.25026/mpc.v14i1.544Keywords:
Knema pallens W.J.deWilde, TLC, Penggugut Leaves, DPPHAbstract
Penggugut (Knema pallens W.J. deWilde) is an endemic plant from Kalimantan belongs to Myristicaceae or the nutmeg family. Penggugut can be found in habitats like lowland forest, sandy forests and mangroves. The efficacy and activity of penggugut especially the leaves, have not been widely known or studied. So that make the purpose was to know about the percentage yield of extract and fraction from penggugut leaves and to know the activity of Radical Scavenging DPPH from penggugut leaves. The methods used include extraction, liquid-liquid fractination and TLC by spraying DPPH. The results there are percentage of yield : methanol extract is 8,04%, n-hexane fraction is 28,9%, ethyl acetate fraction is 7,83% and n-butanol fraction is 9,8%. In the qualitative test, it was found that the spots have activity of Radical Scavenging were marked with yellow spots on a purple background, there are in the methanol extract have 4 spots with Rf values (0,07; 0,14; 0,29; 0,43), the n-hexane fraction have 3 spots with Rf values ??(0,1; 0,21; 0,43), the ethyl acetate fraction have 5 spots with Rf values ??(0,07; 0,17; 0,29; 0,43; 0,53) and the n-butanol fraction did not get any spots.
References
Akasia Alanis Ismi, I Dewa Nyoman Nurweda Putra, dan I Nyoman Giri Putra. 2021. Skrining Fitokimia Ekstrak Daun Mangrove Rhizophora mucronata dan Rhizophora apiculata yang Dikoleksi dari Kawasan Mangrove Desa Tuban, Bali. Journal Of Marine Research And Technology vol. 4 (1), 16-22.
W. J. J. O. DeWilde. 2000. Flora Malesiana Series I - Seed Plants Myristicaceae. Nationaal Herbarium Nederland vol. 14.
Roanisca Occa dan Robby Gus Mahardika. 2021. Skrining Fitokimia dan Antibakteri Ekstrak Etanol Daun Binjai (Mangiferacaesia) Terhadap Bakteri Escherichia coli. Jurnal Pharmascience, vol. 8 (2), 9–16.
Salleh Wan Mohd Nuzul Hakimi Wan dan Farediah Ahmad. 2017. Phytochemistry and biological activities of the genus Knema (Myristicaceae). Pharmaceutical Sciences, vol. 23, 249-255.
Sukmawaty Eka, H. Hafsan, Mashuri Masri, Inna Shintia, Sinar Wahyuni, dan Ulfa Nur Alfriani Amir. 2021. Skrining Fitokimia Dan Aktivitas Antioksidan Ekstrak Etil Asetat Cendawan Endofit Aspergillus sp. Jurnal Biotik vol. 8 (2),218-231.
Erlidawati, Safrida, & Mukhlis. 2018. Potensi Antioksidan sebagai Antidiabetes (1st ed.). Syiah Kuala University Press
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Proceeding of Mulawarman Pharmaceuticals Conferences
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.