Uji Toksisitas Ekstrak Berbasis Nades dari Daun Kadamba (Mitragyna Speciosa Korth) terhadap Mencit (Mus Musculus)

Toxicity of Nades-Based Extract From Kadamba Leaf (Mitragyna Speciosa Korth) on Mice (Mus Musculus)

Authors

  • Fauzan Afandi Laboratorium Penelitian dan Pengembangan Kefarmasian “Farmaka Tropis”, Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia
  • Vita Olivia Siregar Laboratorium Penelitian dan Pengembangan Kefarmasian “Farmaka Tropis”, Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia
  • Islamudin Ahmad Laboratorium Penelitian dan Pengembangan Kefarmasian “Farmaka Tropis”, Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

DOI:

https://doi.org/10.25026/mpc.v14i1.555

Keywords:

Toxicity, Kadamba leaf, Mus musculus

Abstract

Traditionally, kadamba leaves have benefits for treating diarrhea, diabetes, pain, rheumatism, gout and have been scientifically proven to be antinociceptive and inhibit T47D breast cancer cells. This study aims to determine the toxicity of NADES-based extract from kadamba leaves by looking at the LD50 value and its effect on body weight and behavior of mice. The 15 mice used were divided into 5 groups with 3 mice in each group. The NADES-based extract from kadamba leaves was given orally with various doses of 4000 mg/KgBB, 8000 mg/KgBB, 16000 mg/KgBB, and 32000 mg/KgBB and one group was given a solvent. Each group was observed for 30, 60, 120, 180, and 240 minutes after administration of the extract and the observations were continued for 14 days to observe toxic symptoms, changes in body weight and death. Based on the results of the study, no deaths were found in the solvent group, 4000 mg/KgBB, and 8000 mg/KgBB. For the group with a dose of 16000 mg/KgBW, 2 mice died and the group with a dose of 32000 mg/KgBW found the death of 3 mice, so the LD50 value obtained was 14.256 g/KgBW which was included in the practically non-toxic category.

References

Jugran AK, Rawat S, Devkota HP, Bhatt ID, Rawal RS. Diabetes and plant-derived natural products: From ethnopharmacological approaches to their potential for modern drug discovery and development. Phyther Res. 2021;35(1):223-245. doi:10.1002/ptr.6821

Mustapa MA, Taupik M, Hanapi F. Uji Praklinik Kombinasi Obat Herbal Kopi Pinogu (Coffea canephora var Robusta) Dan Bunga Cengkeh (Syzygium aromaticum) Terhadap Penurunan Kadar Asam Urat Pada Mencit. Parapemikir J Ilm …. 2019;8(2):14-20. https://repository.ung.ac.id/get/karyailmiah/3900/uji-praklinik-kombinasi-obat-herbal-kopi-pinogu-coffea-canephora-var-robusta-dan-bunga-cengkeh-syzygium-aromaticum-terhadap-penurunan-kadar-asam-urat-pada-mencit.pdf

Tran N, Pham B, Le L. Bioactive Compounds in Anti-Diabetic Plants?: Biology (Basel). 2020;9(252):1-31.

Yakubu OE, Imo C, Shaibu C, Akighir J, Ameh DS. Effects of Ethanolic Leaf and Stem-bark Extracts of Adansonia digitata in Alloxan-induced Diabetic Wistar Rats. J Pharmacol Toxicol. 2019;15(1):1-7. doi:10.3923/jpt.2020.1.7

WHO. WHO Lists Two Additional COVID-19 Vaccines for Emergency Use and COVAX Roll-Out. WHO.int. Published 2021. Accessed June 10, 2021. https://www.who.int/news/item/15-02-2021-who-lists-two-additional-covid-19-vaccines-for-emergency-use-and-covax-roll-out

Meles DK. Peran Uji Praklinik Dalam Bidang.; 2010.

Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional. Laporan Nasional Riset Khusus Eksplorasi Pengetahuan Lokal Etnomedisin dan Tumbuhan Obat Berbasis Komunitas di Indonesia (RISTOJA) Tahun 2015. Balai Besar Besar Penelit dan Pengemb Tanam Obat. Published online 2015:69. http://labdata.litbang.depkes.go.id/riset-badan-litbangkes/menu-riskesnas/menu-rikus/418-rikus-ristoja-2015

Ismail I, Wahab S, Sidi H, Das S, Lin LJ, Razali R. Kratom and Future Treatment for the Opioid Addiction and Chronic Pain: Periculo Beneficium? Curr Drug Targets. 2017;20(2):166-172. doi:10.2174/1389450118666170425154120

Veltri C, Grundmann O. Current perspectives on the impact of Kratom use. Subst Abuse Rehabil. 2019;Volume 10:23-31. doi:10.2147/sar.s164261

Luliana S, Islamy MR. Aktivitas Antinosiseptif Fraksi Diklorometana Daun Kratom (Mitragyna speciosa Korth.) Rute Oral Pada Mencit Jantan Swiss. Pharm Sci Res. 2018;5(2):58-64. doi:10.7454/psr.v5i2.3895

Tiaravista AG, Robiyanto R, Luliana S. Aktivitas Antinosiseptif Fraksi N-Heksan Daun Kratom (Mitragyna Speciosa Korth.) Melalui Rute Oral Pada Mencit Jantan Swiss. Farmaka. 2019;17:40-51.

Ikhwan D, Harlia, Widiyantoro A. Karakterisasi Senyawa Sitotoksik Dari Fraksi Etil Asetat Daun Kratom (Mitragyna speciosa Korth.) dan Aktivitasnya Terhadap Sel Kanker Payudara T47D. J Kim Khatulistiwa. 2018;7(2):18-24.

Paulucci VP, Couto RO, Teixeira CCC, Freitas LAP. Optimization of the extraction of curcumin from Curcuma longa rhizomes. Rev Bras Farmacogn. 2013;23(1):94-100. doi:10.1590/S0102-695X2012005000117

Choi YH, van Spronsen J, Dai Y, et al. Are natural deep eutectic solvents the missing link in understanding cellular metabolism and physiology? Plant Physiol. 2011;156(4):1701-1705. doi:10.1104/pp.111.178426

Dai Y, van Spronsen J, Witkamp GJ, Verpoorte R, Choi YH. Natural deep eutectic solvents as new potential media for green technology. Anal Chim Acta. 2013;766:61-68. doi:10.1016/j.aca.2012.12.019

Vanda H, Dai Y, Wilson EG, Verpoorte R, Choi YH. Green solvents from ionic liquids and deep eutectic solvents to natural deep eutectic solvents. Comptes Rendus Chim. 2018;21(6):628-638. doi:10.1016/j.crci.2018.04.002

Raza M, Al-Shabanah OA, El-Hadiyah TM, Al-Majed AA. Effect of prolonged vigabatrin treatment on hematological and biochemical parameters in plasma, liver and kidney of Swiss albino mice. Sci Pharm. 2002;70(2):135-145. doi:10.3797/scipharm.aut-02-16

Jothy SL, Zakaria Z, Chen Y, Lau YL, Latha LY, Sasidharan S. Acute oral toxicity of methanolic seed extract of Cassia fistula in mice. Molecules. 2011;16(6):5268-5282. doi:10.3390/molecules16065268

Downloads

Published

2021-12-31

How to Cite

Afandi, F., Siregar, V. O., & Ahmad, I. (2021). Uji Toksisitas Ekstrak Berbasis Nades dari Daun Kadamba (Mitragyna Speciosa Korth) terhadap Mencit (Mus Musculus): Toxicity of Nades-Based Extract From Kadamba Leaf (Mitragyna Speciosa Korth) on Mice (Mus Musculus). Proceeding of Mulawarman Pharmaceuticals Conferences, 14(1), 190–195. https://doi.org/10.25026/mpc.v14i1.555

Most read articles by the same author(s)

1 2 > >>