Uji Antioksidan Ekstrak Metanol Kulit Batang Pule (Alstonia scholaris) khas Kalimantan dengan Metode DPPH

Antioxidant Test of Methanol Extract of Pule Bark (Alstonia scholaris) typical of Kalimantan with the DPPH Method

Authors

  • Nur Halimah Laboratorium Penelitian dan Pengembangan Kefarmasian “Farmaka Tropis”, Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia
  • Mahfuzun Bone Laboratorium Penelitian dan Pengembangan Kefarmasian “Farmaka Tropis”, Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia
  • Fajar Prasetya Laboratorium Penelitian dan Pengembangan Kefarmasian “Farmaka Tropis”, Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

DOI:

https://doi.org/10.25026/mpc.v14i1.576

Keywords:

Antioxidant, Methanol Extract, Pule Bark, DPPH

Abstract

Pule (Alstonia scholaris) is one of the plants that are empirically often used in medicine. Pule bark contains flavonoids which can generally be used as antioxidants. This study aims to determine the content of secondary metabolites and antioxidant activity of the methanol extract of pule bark by determining the IC50 value of the extract. The secondary metabolite test was carried out by reacting the extract with phenolic test reagents, steroids, terpenoids, saponins, flavonoids and alkaloids. The antioxidant activity test was carried out using the DPPH method (2,2-Diphenyl-1-Picrylhydrazil). Antioxidant activity was measured by the ability of the methanol extract of Pule bark to reduce DPPH which was measured using a UV-Vis Spectrophotometer so that absorbance data was obtained and then processed by linear regression method. The results showed that the extract contained phenolic and flavonoid compounds. While the DPPH test showed that the extract had weak antioxidant activity with an IC50 value of 472.473 ppm.

References

Pratiwi Dr, Bintang M, Simanjuntak P. 2014. Lelutung Tokak (Tabernaemontana Macrocarpa Jack). As Source Of Bioactive Substances, Antioxidant And Anticancer ). J Ilmu Kefarmasian Indonesia. 12 (6), 267–72.

Winarsih H. 2007. Antioksidan Alami Dan Radikal Bebas.

Samirana Po, Putra Pas, Leliqia Npe.2017. Uji Penangkapan Radikal 2,2-Difenil-1- Pikrihidrazil Dan Profil Bioautografi Ekstrak Etanol Kulit Batang Bidara (Ziziphus Mauritiana Auct. Non Lamk.). Jurnal Farmasi Udayana. 6 (1), 55-61

Arief, Aziz.2019. Uji Efek Antipiretik Ekstrak Daun Pule (Alstonia scholaris) pada Mencit (Mus musculus). Jurnal Kesehatan. 3 (2) : 142

Mardiyah, U., Fasya, G. A. Fauziyah, B., dan Amalia, S. 2014. Ekstraksi Uji Aktivitas Antioksidan dan Identifikasi Golongan Senyawa Aktif Alga Merah Eucheuma spinosum dari Perairan Banyuwangi. Jurnal Achemy. 3 (1) : 42

Sa’adah, H., Nurhasnawati, H. 2015. Perbandingan Pelarut Etanol Dan Air Pada Pembuatan Ekstrak Umbi Bawang Tiwai (Eleutherine Americana Merr) Menggunakan Metode Maserasi. Jurnal Ilmiah Manuntung. 1(2) : 149-153.

Muthmainnah, B. 2017. Skrining Fitokimia Senyawa Metabolit Sekunder dari Ekstrak Etanol Buah (Punica granatum L.) dengan Metode Uji warna. Media Farmasi. 8 (2) : 26

Paricia, S, Meiske, S, dan Lidya, I. 2020. Uji Senyawa Fitokimia dan Aktivitas Antioksidan Tanaman Patah Tulang (Euphorbia tirucalli L.).Jurnal MIPA. 9 (2) : 64-69

Frelinsia, V.M, Defny, S, dan Irma, A.2020. Uji Aktivitas Antioksidan Ekstrak Etanol Ascidian Herdmania Momus dengan Metode DPPH (2,2-Diphenyl-1-Picrylhydrazil). Jurnal MIPA. 9 (3) : 29-34

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Published

2021-12-31

How to Cite

Halimah, N., Bone, M., & Prasetya, F. (2021). Uji Antioksidan Ekstrak Metanol Kulit Batang Pule (Alstonia scholaris) khas Kalimantan dengan Metode DPPH: Antioxidant Test of Methanol Extract of Pule Bark (Alstonia scholaris) typical of Kalimantan with the DPPH Method. Proceeding of Mulawarman Pharmaceuticals Conferences, 14(1), 90–95. https://doi.org/10.25026/mpc.v14i1.576

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